Gel Electrophoresis Buffers and Diluents
Biotium 6X GelRed Prestain Buffer with Orange Tracking Dye
GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer, and eliminates the need to add fluorescent DNA dye to the agarose gel during casting. This prestain buffer contains contains an orange electrophoresis tracking dye that runs at approximately 50 bp in a 1% agarose gel. Properties: Liquid; Store at 4°C and protect from light.
Electron Microscopy Sciences Acetate Buffer 500 ML
Used in Vogt's Method for Nerve Cell Products. AFIP, Manual of Histological Staining Methods, 3rd Ed., Ed. L. Luna: New York: McGraw-Hill Publications, c. 1968, p. 212
Biotang Inc RNAse Free Sodium Acetate, 1M, pH 4.5, 100 ml
RNAse Free Sodium Acetate, 1M, pH 4.5, 100 ml.
Bioworld Loading Buffer, 15% Ficoll (6x), 5 mL
- Ultra Pure Grade - For nucleic acid applications. Absent of RNase and DNase activity. Abs.@525nm (1:500, Water)- 0.3-0.6 Abs.@588-594nm (1:500, Water)- 0.5-0.8 Abs.@635-641nm (1:500, Water)- 0.5-0.7 Clarity(P/F)- Pass DNase(P/F)- None Expiration Date - Report Protease(P/F)- None RNase(P/F)- None
Zymo Research Corporation RNA Pre-Wash Buffer, 20 ml
RNA Pre-Wash Buffer (25 ml). Sold individually or as part of a nucleic acid purification kit for the purificaiton of RNA.
Biotang Inc RNAse Free Sodium Acetate, 3M, pH 5.2, 100 ml
RNAse Free Sodium Acetate, 3M, pH 5.2, 100 ml.
New England Biolabs, Inc. T4 DNA Ligase Reaction Buffer – 5 ml
T4 DNA Ligase is the industry standard for performance and quality. New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
Electron Microscopy Sciences Acetate Buffer, 0.2M, pH 3.6-5.6 500 ML
Acetic Acid and Sodium Acetate, 0.2M, pH range 3.6-5.6. Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged. Desired pH should be specified when ordering.
pH: 3.6-5.6
New England Biolabs, Inc. Gel Loading Dye Purple (6X) – 4 ml
Gel Loading Dye, Purple (6X) is the premier gel loading dye from NEB for sharp, tight bands. It is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. The dye also contains Ficoll, which creates brighter and tighter bands when compared to glycerol loading dyes. Attention SYBR Safe and GelRed dye users: Due to an increased concentration of SDS in B7024S, NEB recommends using Gel Loading Dye, Purple, No SDS (6X) (NEB# B7025S) instead.
Bioworld IEF Sample Buffer (4X), 50 mL
Isoelectrofocusing Sample Buffer (4X) Sample buffer used in protein analysis protocols Technical Information:4X Concentration Contains: 20 mM Lysine, free base, 15% Glycerol